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In Vitro
Assay Services

We have the expertise to perform a wide range of assays designed to support your research needs, as well as the experience and the creativity to develop assays to meet your specific unique requirements.
Feel free to contact us for an initial consultation.

Cell-Based Assays

Cell types

  • Immortal cell lines (haematopoietic cells or tumor cells)

  • Primary human cells; PBL, PBMC, neutrophils, purified T cells, purified B cells

  • Primary mouse cells; spleen lymph node, Peyer's patch, peritoneal exudates

Assays

  • Stimulus-induced cytokine production

  • Stimulus-induced signal transduction pathway analysis (phosphorylation)

  • T cell proliferation (CD3/CD28, MLR, SEB)

  • Neutrophil extracellular trap (NET) formation

  • Phagocytosis (fluorescent beads with or without opsonization)

  • Respiratory burst

  • DC generation/antigen presentation / T cell proliferation

  • MAPPS (MHC-associated peptide proteomics)

We are not limited to these! Tell us what you need and we can add something new.

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ELISAs

  • Traditional cytokine ELISAs (TNF-α, IL-6, IL-4, IL-13, IL-5, IL-17α, IFNγ, IFNα/β, etc.)

  • Autoantibody ELISAs (ACPA, anti-collagen antibodies, anti-dsDNA, anti-OVA lgE, etc.)

  • Custom ELISAs with commercially available antibodies

  • Custom ELISAs with new mAb generation in house

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Biochemical Assays

  • SDS-PAGE / Western blots

  • Enzyme assays

    • PAD-mediated citrullination

    • Transglutaminase 2 (TG2)

    • cGAMP/cGAS

    • Caspase activity assays

We are not limited to these! Tell us what you need and we can add something new.

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Flow Cytometry

  • FACSCalibur 4-color flow cytometry

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